Sulfathiazole (CAS 72-14-0) is a small-molecule inhibitor targeting dihydropteroate synthase (DHPS) It is designed to competitively inhibit bacterial folic acid synthesis thereby disrupting the folate pathway and impeding microbial growth Sulfathiazole exerts its biological activity primarily through inhibition of DHPS In in vitro studies sulfathiazole demonstrates bacteriostatic inhibition with IC50 values ranging from low to medium micromolar concentrations depending on specific bacterial strains and experimental conditions Based on these pharmacological properties sulfathiazole holds research potential in investigating antibacterial resistance metabolic pathways of pathogens and enzyme-substrate interactions in microbiological and biochemical research